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  • 标题:Comparison of Two Antigens for Diagnosis of Trichinellosis in Pigs
  • 本地全文:下载
  • 作者:Soad E. Hassan ; FaragalLa M. El-Moghazy ; Nagwa I. Toaleb
  • 期刊名称:World Applied Sciences Journal
  • 印刷版ISSN:1818-4952
  • 电子版ISSN:1991-6426
  • 出版年度:2010
  • 卷号:8
  • 期号:04
  • 出版社:International Digital Organization for Scientific Information Publications
  • 摘要:

    Due to its role in human Trichinellosis, there are increasing global requirements for reliable diagnostic
    method for Trichinella spiralis infection in pigs. Consequently, there is a need for selection of potent
    diagnostic antigen that could be successfully utilized in the diagnosis. Currently, two antigens (somatic and
    excretory-secretory antigens of T. spiralis larvae) were evaluated for the diagnosis of pig trichinellosis using
    ELISA. The ES antigen exhibited highest diagnostic potential (88.3%) as compared to somatic antigen (68.3%).
    The diagnostic potential of ES antigen is attributed to five immunogenic bands identified by infected pig sera
    in immunoblot. These bands are of molecular weight 127, 58, 35, 20.5 and 12 KDa. While in somatic antigen six
    immunogenic bands of molecular weight 230, 89, 58, 27, 15 and 6 KDa were identified. In conclusion, monitoring
    for T. spiralis antibodies in swine farms should be based on screening pig sera by means of ELISA in
    which larval ES antigen was utilized.

  • 关键词:Trichinella spiralis ; Diagnosis ; ELISA ; Excretory-secretory antigen ; Somatic antigen; Immunoblot
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