文章基本信息
- 标题：Prevalence of Tick-borne Encephalitis Virus ( T B E V) in Samples of Raw Milk Taken Randomly from Cows, Goats and Sheep in Eastern Poland
- 本地全文：下载
- 作者：Ewa Cisak ; Angelina Wojcik-Fatla ; Violetta Zajac 等
- 期刊名称：Annals of Agricultural and Environmental Medicine
- 印刷版ISSN：1232-1966
- 电子版ISSN：1898-2263
- 出版年度：2010
- 卷号：17
- 期号：02
- 出版社：Institute of Agricultural Medicine in Lublin
- 摘要：
A total of 119 unpasteurized milk samples taken from 63 cows, 29 goats and
27 sheep bred on 8 farms situated on the territory of the Lublin province (eastern Poland),
an area of risk of tick-borne encephalitis (TBE), were examined for the presence of RNA
of tick-borne encephalitis virus (TBEV) by the nested RT-PCR method. Milk samples
were also tested for the presence of anti-TBEV antibodies by ELISA test. By RT-PCR,
the greatest prevalence of TBE virus was found in the milk of sheep (22.2%), followed
by milk of goats (20.7%) and cows (11.1%). By ELISA, the greatest prevalence of anti-
TBEV antibodies was found also in the milk of sheep (14.8%), followed by milk of cows
(3.2%) and goats (0%). The results suggest a potential risk of infection with TBEV by
drinking raw milk on endemic areas of TBE, and indicate a need for milk pasteurization
before consumption - 关键词：tick-borne encephalitis virus; raw milk; RT-PCR; antibodies
