期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：1999
卷号：6
期号：02
出版社：Institute of Agricultural Medicine in Lublin
摘要： Previous studies have proposed that increasing the temperature around the rat isolated ileal muscles induces a heat-released substance (HRS) which causes a decrease in the contractile response. Therefore, we have studied various characteristics of this proposed HRS in animals exposed to heat-stress conditions. In these animals, the contraction of muscles in response to carbachol was decreased by increasing the temperature from 37°C to 40°C. Further, bathing the ileum in a conditioned medium prepared by incubation of the ileal muscle at 40°C has shown less contraction at 40°C than in presence of normal Krebs medium. In addition, conditioned mediums, prepared at 40°C were run on SDS-polyacrylamide gel electrophoresis and have shown the presence of a distinct protein band at a m.w. region of 55 kDa. These results confirm our previous studies that increasing the temperature around the muscles induces the HRS that causes the decrease in the contractile response.
关键词：words: heat illnesses; muscle contraction; temperature effect; rat
