According to many research findings, the presence of discourse markers (DMs) enhances readers’ comprehension of the texts they read. However, there is a paucity of research on the relationship between knowledge of DMs and reading comprehension (RC) and the present study explores the relationship between them. Knowledge of DMs is measured through examining the subjects’ recognition of DMs. To carry out the research, 86 Iranian sophomores majoring in English took a test of DMs alongside a RC test. The correlation between their scores on the two tests was calculated using the software SPSS. The analysis revealed that there is high correlation between the students’ knowledge of DMs (i.e., their correct recognition of discourse markers) and their reading comprehension (rxy = .71). Moreover, high correlation carries a strong regression power and scores on a test of DMs could be a good indicator of the test takers’ reading ability.