期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：2
页码：671-675
DOI：10.1073/pnas.0307681100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The association of Ca2+-activated K+ channels with voltage-gated Ca2+ channels at the presynaptic active zones of hair cells, photoreceptors, and neurons contributes to rapid repolarization of the membrane after excitation. Ca2+ channels have been shown to bind to a large set of synaptic proteins, but the proteins interacting with Ca2+-activated K+ channels remain unknown. Here, we report that the large-conductance Ca2+-activated K+ channel of the chicken's cochlear hair cell interacts with {beta}-catenin. Yeast two-hybrid assays identified the S10 region of the K+ channel's {alpha}-subunit and the ninth armadillo repeat and carboxyl terminus of {beta}-catenin as necessary for the interaction. An antiserum directed against the {alpha}-subunit specifically coprecipitated {beta}-catenin from brain synaptic proteins. {beta}-Catenin is known to associate with the synaptic protein Lin7/Velis/MALS, whose interaction partner Lin2/CASK also binds voltage-gated Ca2+ channels. {beta}-Catenin may therefore provide a physical link between the two types of channels at the presynaptic active zone.
