期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：24
页码：8945-8950
DOI：10.1073/pnas.0400588101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nitric oxide (NO) possesses antiinflammatory effects, which may be exerted via its ability to inhibit the transcription factor, NF-{kappa}B. A commonly proposed mode of action for inhibition of NF-{kappa}BbyNO involves interference with NF-{kappa}B binding to DNA. Because activation of inhibitory {kappa}B kinase (IKK), the prerequisite enzyme complex necessary to induce NF-{kappa}B, is subject to redox regulation, we assessed whether IKK could present a more proximal target for NO to inhibit NF-{kappa}B activation. We demonstrate here that S-nitrosothiols (SNO) caused a dose-dependent inhibition of the enzymatic activity of IKK, in lung epithelial cells and in Jurkat T cells, which was associated with S-nitrosylation of the IKK complex. Using biotin derivatization of SNO, we revealed that IKK{beta
