期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：45
页码：15915-15920
DOI：10.1073/pnas.0405904101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Both turn sequence and interstrand hydrophobic side-chain-side-chain interaction have been suggested to be important determinants of {beta}-hairpin stability. However, their roles in controlling the folding dynamics of {beta}-hairpins have not been clearly determined. Herein, we investigated the structural stability and folding kinetics of a series of tryptophan zippers by static IR and CD spectroscopies and the IR temperature jump method. Our results support a {beta}-hairpin folding mechanism wherein the rate-limiting event corresponds to the formation of the turn. We find that the logarithm of the folding rate depends linearly on the entropic change associated with the turn formation, where faster folding correlates with lower entropic cost. Moreover, a stronger turn-promoting sequence increases the stability of a {beta}-hairpin primarily by increasing its folding rate, whereas a stronger hydrophobic cluster increases the stability of a {beta}-hairpin primarily by decreasing its unfolding rate.
