期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2005
卷号：102
期号：22
页码：7934-7939
DOI：10.1073/pnas.0500357102
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Adoptive transfer of antigen-specific CD4+ and CD8+ T cells is one of the most efficient forms of cancer immunotherapy. However, the isolation of antigen-specific CD4+ T cells is limited because only few tumor-associated helper epitopes are identified. Here, we used T cell antigen receptor gene transfer to target CD4+ T cells against an MHC class I-presented epitope of a model tumor antigen. IFN-{gamma}-producing CD4+ T cells were unable to expand in vivo and to provide help for tumor rejection. In contrast, CD4+ T cells producing high levels of IL-2 expanded in vivo, provided help for cytotoxic T lymphocyte-mediated tumor rejection, and developed T cell memory. The data demonstrate in vivo synergy between T cell antigen receptor-transduced CD4+ and CD8+ T cells specific for the same epitope resulting in long-term tumor protection.
关键词：T cell antigen receptor gene transfer ; tumor immunotherapy
