期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：2
页码：583-587
DOI：10.1073/pnas.87.2.583
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The utility and power of 3H NMR spectroscopy as a technique for monitoring biological systems in vivo is illustrated with glucose metabolism in erythrocytes. Use of C-1-tritiated glucose allowed us to monitor the disappearance of the alpha and beta tritons, with the production of lactate and 1H3HO (HTO), as well as some intermediates. Spin lattice relaxation times (T1) were measured to avoid T1 distortion of the spectral intensities. Detection of the formation of 1 mM tritiated water in the presence of 110 M H2O protons and deuterons allows the eventual fate of the label in the pentose shunt to be observed in vivo.
