期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：7
页码：2657-2661
DOI：10.1073/pnas.87.7.2657
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The decrease in overall translation activity occurring concomitantly with the transition from the exponential growth phase to the stationary phase of Escherichia coli cells was found to be accompanied by the appearance of 100S ribosomes (dimers of 70S ribosome monomers). Analysis of ribosomal proteins by the radical-free and highly reducing method of two-dimensional gel electrophoresis indicated that a protein, designated protein E, was exclusively associated with 100S ribosomes. From the results, we propose that protein E is a "ribosome modulation factor" (RMF), which associates with 70S ribosomes and converts them to a dimeric form. A homology search of the partial amino acid sequence of RMF using the DNA sequence data bases revealed that the rmf gene, which encodes RMF, is located next to the fabA gene at 21.8 min on the E. coli chromosome.
