期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：14
页码：5278-5282
DOI：10.1073/pnas.87.14.5278
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have isolated rat brain cDNA clones encoding AE2, a homologue of the erythrocyte anion exchanger, band 3 (AE1). Immunocytochemistry and in situ hybridization reveal that, in brain, AE2 expression is restricted to the basolateral membrane of the choroid plexus epithelium. Expression of a full-length mouse AE2 cDNA in COS-7 cells resulted in chloride- and bicarbonate-dependent alterations in intracellular pH, demonstrating that AE2 is a Cl/HCO3 exchanger. Cation replacement studies indicate that AE2-mediated exchange is independent of extracellular sodium. COS-7 cells expressing a mutant rat AE2 cDNA clone that lacks the cytoplasmic NH2-terminal 660 amino acids exhibit identical responses to cation and anion substitution. These results indicate that this domain does not play a significant role in either correct insertion of the transporter into the plasma membrane or anion exchange.
