期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：14
页码：5331-5335
DOI：10.1073/pnas.87.14.5331
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downstream basic region that may make up a DNA-binding domain. The zinc-finger domain of the NIT2 protein was synthesized in two ways to examine its DNA-binding activity with gel-band-mobility shift and DNA-footprint experiments. The NIT2 protein binds to specific DNA recognition elements that are located upstream of nitrogen-regulated structural genes. Each recognition element contains at least two copies of a core sequence whose consensus is TATCTA.
