标题：Distribution and self-organization of photosynthetic pigments in micelles: implication for the assembly of light-harvesting complexes and reaction centers in the photosynthetic membrane.
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：14
页码：5430-5434
DOI：10.1073/pnas.87.14.5430
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The addition of bacteriochlorophylls and bacteriopheophytins to formamide/water, 3:1 (vol/vol), (or water) containing small spherical micelles of Triton X-100 leads to the reorganization of the detergent into micelles that consist of 5000-40,000 amphiphilic molecules. The pigment distribution within the micelles was determined by modified Poisson statistics taking into consideration the various sizes of micelles. Pigment dimerization occurred in micelles with more than a single occupant and was driven by a free-energy change of -4.5 kcal/mol (1 cal = 4.184 J) for bacteriochlorophyll a in formamide/water, -7.6 kcal/mol for bacteriopheophytin a in formamide/water, and -6.6 kcal/mol for bacteriopheophytin a in water. These values correspond to the room temperature equilibrium constants 2.2 x 10(3) M-1, 3.9 x 10(5) M-1, and 7.5 x 10(4) M-1, respectively. The incorporation of bacteriochlorophylls with attached small formamide polymers and the subsequent dimerization of these pigments in the lipid phase provide a model for studying the synergetic organization of polypeptides and bacteriochlorophyll clusters in the photosynthetic membrane.
