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  • 标题:Characterization of a beta subunit of the gastric H+/K(+)-transporting ATPase
  • 本地全文:下载
  • 作者:M A Reuben ; L S Lasater ; G Sachs
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1990
  • 卷号:87
  • 期号:17
  • 页码:6767-6771
  • DOI:10.1073/pnas.87.17.6767
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The catalytic subunit of the H+/K(+)-transporting ATPase (EC 3.6.1.3 ) has 62% identity to the alpha, or catalytic subunit, of the Na+/K(+)-transporting ATPase (EC 3.6.1.37 ); however, a homologous beta subunit was unknown until recently. Removal of the carbohydrate from purified hog H+/K(+)ATPase vesicles reveals a 35-kDa peptide that, when fragmented with protease V8, gives sequences homologous to both beta 1 and beta 2 subunits of the Na+/K(+)-ATPase. cDNA clones for a beta subunit of the gastric H+/K(+)-ATPase were isolated from a rabbit stomach cDNA library by using degenerate 17-mer oligonucleotide probes made to the protease V8-treated peptides. An open reading frame (54-926) encodes a predicted 291-amino acid peptide with Mr = 33,320, which exhibits 31% and 44% homologies to the Na+/K+)-ATPase beta 1 and Na+/K(+)-ATPase beta 2 proteins, respectively. A Kyte-Doolittle hydropathy plot predicts a single N-terminal transmembrane domain with a small hydrophobic region near the C terminus. The presumed extracytosolic domain contains seven potential N-linked glycosylation sites and six out of nine cysteines. Northern (RNA) blot analysis of stomach RNA with the rabbit H+/K(+)-ATPase beta probe identifies a single mRNA of 1.3-1.5 kilobases, similar in concentration to the alpha subunit mRNA. The presence of a defined gastric H+/K(+)-ATPase beta subunit extends the homology between H+/K(+)-ATPase and the Na+/K(+)-ATPase subclass of phosphoenzyme transport ATPases and distinguishes them from the monomeric Ca2+ and proton pump subclasses.
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