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  • 标题:Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1
  • 本地全文:下载
  • 作者:P M Handley ; M Mueckler ; N R Siegel
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1991
  • 卷号:88
  • 期号:1
  • 页码:258-262
  • DOI:10.1073/pnas.88.1.258
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.
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