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  • 标题:In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides
  • 本地全文:下载
  • 作者:R V Zackroff ; W W Idler ; P M Steinert
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1982
  • 卷号:79
  • 期号:3
  • 页码:754-757
  • DOI:10.1073/pnas.79.3.754
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein.
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