期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：3
页码：758-761
DOI：10.1073/pnas.79.3.758
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：An immunological procedure that uses antiserum against homogeneous calf thymus DNA polymerase beta to detect immunoreactive peptides on NaDodSO4/polyacrylamide gel electrophoresis demonstrates a high degree of conservation of protein sequence and molecular weight for this enzyme, from parastic protozoans to man. By renaturation of DNA polymerase activity in situ after electrophoresis, the enzymatically active peptides are shown to correspond to the immunoreactive peptides. The persistence of sequence and molecular weight for the catalytic peptide of DNA polymerase beta through eons of evolutionary time suggests an essential role for this enzyme in DNA metabolism of complex cells.
