期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：3
页码：874-878
DOI：10.1073/pnas.79.3.874
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Genes for naphthalene metabolism are localized on nah7, an 83-kilobase (kb) plasmid, in two gene clusters under salicylate control. Polar mutations formed by insertion of the transposon Tn5 permit detection of the transcription direction and the gene organization within two approximately 10-kb DNA segments separated by a approximately 7-kb regulatory gene region. The gene cluster specifying conversion of naphthalene to salicylate lies near the left initiation of a 25-kb DNA fragment A released by EcoRI; that for the salicylate pathway via catechol meta-fission lies near the right terminus with extension into the adjoining 5.9-kb fragment C. The genetic organization and regulation resemble the tol plasmid-encoded "upper" and "lower" pathways of toluene/xylene oxidation in Pseudomonas putida mt2.
