期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：4
页码：1254-1258
DOI：10.1073/pnas.79.4.1254
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A monoclonal T-cell-derived suppressor factor specific for the terpolymer poly(LGlu60LAla30LTyr10) produced by the T-cell hybridoma 258 C4.4, was purified to homogeneity. This was accomplished by fractionation of the culture medium by using a combination of affinity chromatography and reverse-phase and ion-exchange high-performance liquid chromatography. The purified factor is composed of a single Mr 24,000 polypeptide chain, and the homogeneous protein maintains the ability to suppress antibody and T-cell proliferative responses to poly(LGlu60LAla30LTyr10) specifically. The specific activity of pure suppressor factor is calculated to be 8 X 10(7) units/micrograms.
