期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：5
页码：1388-1392
DOI：10.1073/pnas.79.5.1388
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Point mutations have been introduced in vitro in a cloned nematode tRNAPro gene. Four different mutant clones altered in the DNA that codes for tRNAPro have been isolated. Studies on the expression of the mutant genes by microinjection into Xenopus oocyte nuclei reveal that their activities as transcription templates are reduced. The results show that DNA sequences that code for the extra arm and the stem of the T-psi-C-G arm of the tRNAPro have an important role in the initiation of tRNA gene transcription.
