期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：11
页码：3547-3550
DOI：10.1073/pnas.79.11.3547
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Immunocytochemistry with a specific antiserum and protein A-horseradish peroxidase permits visualization of the sites of gamma-glutamyltransferase [(5-glutamyl)-peptide:amino-acid 5-glutamyltransferase, EC 2.3.2.2 ] at both the light- and the electron-microscope levels. As seen by light microscopy, the enzyme is localized only in the proximal convolutions of the renal tubules. Electron microscopy reveals dense deposits of 3,3'-diaminobenzidine reaction product embedded in the glycocalyx along the entire luminal surface of the microvilli and in the basolateral membranes.
