期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：11
页码：3666-3670
DOI：10.1073/pnas.79.11.3666
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Acetylcholine receptor-phosphorylation has been compared in sealed and lysed right-side-out membrane vesicles prepared form Torpedo californica electric organ. Phosphorylation was increased 5- to 12-fold in hypotonically lysed vesicles as compared with untreated vesicles. Control experiments confirm that this enhancement is a result of increased permeability of the membrane to ATP. These data suggest that the acetylcholine receptor kinase is located on the cytoplasmic side of the plasma membrane. Results with detergent lysis support this conclusion. Although the acetylcholine receptor constitutes less than 10% of the total protein in these membranes, the kinase was found to be highly specific for polypeptides corresponding in molecular weight to acetylcholine receptor subunits.
