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  • 标题:Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes
  • 本地全文:下载
  • 作者:W C Wu ; S I Walaas ; A C Nairn
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1982
  • 卷号:79
  • 期号:17
  • 页码:5249-5253
  • DOI:10.1073/pnas.79.17.5249
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Depolarization-induced calcium influx into rat cerebral cortex synaptosomes increased the phosphorylation of several synaptosomal proteins as examined by 32Pi incorporation. A phosphopeptide mapping technique involving NaDodSO4/polyacrylamide gels has been used to show that phosphorylation of a Mr 87,000 substrate protein is stimulated by depolarization-induced calcium influx. Phosphorylation of this Mr 87,000 substrate occurred in synaptosomal cytosol and was markedly stimulated by calcium/phosphatidylserine. Calmodulin inhibited this phosphorylation reaction. This substrate for calcium/phospholipid-dependent protein kinase is enriched in and appears to be specific to neurons.
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