期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：17
页码：5282-5286
DOI：10.1073/pnas.79.17.5282
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The marked lability in cell-free preparations of the enzyme system involved in cellulose biosynthesis in most organisms studied led us to investigate factors responsible for loss of activity on cellular disruption. Previous studies have led to the suggestion that the existence of a transmembrane electrical potential ({Delta}{Psi}) may be one factor responsible for maintaining an active system in intact cells. In this report, we show that dissipation of the {Delta}{Psi} in metabolizing cells of Acetobacter xylinum results in severe inhibition of cellulose synthesis. The effect can be reversed by restoration of the {Delta}{Psi}. Inhibition of cellulose biosynthesis by dissipation of the {Delta}{Psi} can be observed under conditions in which no substantial impairment of energy metabolism occurs--i.e., under conditions in which a transmembrane pH gradient is of sufficient magnitude to maintain an adequate overall protonmotive force across the membrane. The inhibition of cellulose biosynthesis is specifically related to changes in the {Delta}{Psi
关键词：protonmotive force ; plasma membrane ; glucan ; polysaccharide biosynthesis
