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  • 标题:Reconstruction of the chloroplast noncyclic electron transport pathway from water to NADP with three integral protein complexes
  • 本地全文:下载
  • 作者:Eric Lam ; Richard Malkin
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1982
  • 卷号:79
  • 期号:18
  • 页码:5494-5498
  • DOI:10.1073/pnas.79.18.5494
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Reconstruction of photosynthetic noncyclic electron transport from water to NADP has been accomplished by using three integral protein complexes isolated from chloroplast thylakoid membranes: photosystems I and II and the cytochrome b6-f complex. This system shows an absolute dependence on the presence of all three protein complexes for NADP reduction, in addition to plastocyanin, ferredoxin, and ferredoxin-NADP reductase. The reconstructed system was found to be sensitive to low concentrations of known inhibitors of noncyclic electron transport. Depletion of the Rieske iron-sulfur center and bound plastoquinone from the cytochrome b6-f complex resulted in an inhibition of the photoreduction of NADP.
  • 关键词:photosystem I ; photosystem II ; cytochrome b 6- f
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