期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1982
卷号:79
期号:18
页码:5494-5498
DOI:10.1073/pnas.79.18.5494
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:Reconstruction of photosynthetic noncyclic electron transport from water to NADP has been accomplished by using three integral protein complexes isolated from chloroplast thylakoid membranes: photosystems I and II and the cytochrome b6-f complex. This system shows an absolute dependence on the presence of all three protein complexes for NADP reduction, in addition to plastocyanin, ferredoxin, and ferredoxin-NADP reductase. The reconstructed system was found to be sensitive to low concentrations of known inhibitors of noncyclic electron transport. Depletion of the Rieske iron-sulfur center and bound plastoquinone from the cytochrome b6-f complex resulted in an inhibition of the photoreduction of NADP.
关键词:photosystem I ; photosystem II ; cytochrome b 6- f