期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1983
卷号：80
期号：5
页码：1221-1223
DOI：10.1073/pnas.80.5.1221
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.
