期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1983
卷号：80
期号：5
页码：1401-1405
DOI：10.1073/pnas.80.5.1401
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Recombination between lacZ alleles in crosses of {lambda}lacZ1- x {lambda}lacZ2- and F-lacZ1- x {lambda}lacZ2- in Escherichia coli ({lambda}) can be stimulated manyfold by UV irradiation of one of the {lambda}lacZ phages [Porter, R. D., McLaughlin, T. & Low, B. (1979) Cold Spring Harbor Symp. Quant. Biol. 43, 1043-1048]. Analogous stimulation has now been observed by coinfection of the cells by UV-irradiated {lambda} phage which carries no lac region. This indirect stimulation is not dependent on induction of the SOS system. The bacterial uvr system can effectively remove the damages on the {lambda} DNA which cause the indirect stimulation. Among a number of mutations tested, only ssb-1 was found to cause a drastic decrease in the indirect stimulation. Indirect stimulation was caused only by using phage that had a region of homology with the recombining phage. The homologous region can be separated from the recombining region by an extended nonhomologous region (>7.9 x 103 base pairs). This implies that damages to the DNA molecule, which stimulate recombination, can be located very far from the recombining region of the molecule.
