期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1983
卷号:80
期号:6
页码:1717-1720
DOI:10.1073/pnas.80.6.1717
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:Conditioned medium from cultures of mouse macrophages incubated with endotoxin in a serum-free medium contains an inhibitor of the growth and differentiation of erythroid precursor cells of mouse Friend virus-transformed erythroleukemia cells. Endotoxin itself has no inhibitory effect. The endotoxin-induced macrophage mediator inhibits the growth and differentiation of dimethyl sulfoxide-, hexamethylenebisacetamide-, butyric acid-, and hypoxanthine-induced cells but has no effect on hemin-induced cells. The conditioned medium has its maximal inhibitory effect on committed erythroid precursor cells, a decreased effect on uncommitted stem cells, and no effect on fully differentiated erythroid cells. These results demonstrate that endotoxin stimulation of macrophages leads to the production of a humoral factor(s) which is critical for the growth and differentiation of erythroid precursor cells.