期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1984
卷号：81
期号：6
页码：1859-1863
DOI：10.1073/pnas.81.6.1859
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In search for olfactory receptor molecules, we carried out comprehensive electrophoretic mapping of membrane proteins in the cilia of frog olfactory epithelium. Seven polypeptides, extracted from isolated cilia by nonionic detergent, were unique to the sensory organelles, compared to nonsensory (respiratory) counterparts. Olfactory cilia contained 3-10 times more membrane-associated protein as compared to respiratory cilia, in agreement with reported densities of freeze-fracture intramembranous particles. Four of the olfactory polypeptides were major constituents of the ciliary membrane, each amounting to greater than 10% of its total protein. Three major and one minor specific polypeptide were glycosylated, whereas membranes of nonsensory cilia were practically devoid of glycoproteins. A clear difference in surface composition was also shown by microscopic visualization of fluoresceinated lectin bound to intact isolated cilia. Two of the olfactory glycoproteins displayed pronounced heterogeneity of apparent molecular weight, which could partly be due to protein sequence diversity, as expected for odorant receptor molecules. The recently described inhibition of odorant-evoked sensory potentials by the lectin concanavalin A is consistent with the hypothesis that one or more of the specific glycoproteins described here plays a role in olfactory reception.
