期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1985
卷号：82
期号：9
页码：3045-3049
DOI：10.1073/pnas.82.9.3045
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A monoclonal antibody (against 7A antigen), raised against a homogenate of embryonic rat forebrain, marks the distribution of region- and developmental stage-specific cell-surface antigens in the mammalian central nervous system. In the mouse, immunocytochemical staining revealed that 7A antigen is expressed almost exclusively in germinal layers of the cerebral cortex beginning as early as day 11 of gestation and becomes undetectable by birth. The typical staining is seen at embryonic days 13-15, in which only the ventricular and the subventricular zones of the cerebral cortex are intensely labeled, whereas most other areas of the brain are unlabeled. Tissue culture studies indicate that the 7A antigen is localized at the plasma membrane. Molecular species possessing the antigen are identified as neutral glycolipids that contain the X-determinant trisaccharide: (formula; see text) This glycoconjugate is expressed in embryonic brains of many mammalian species (mouse, rat, rabbit, calf, and human) and may be a cell-surface component important in normal development of the central nervous system.
