期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1985
卷号：82
期号：13
页码：4346-4350
DOI：10.1073/pnas.82.13.4346
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nuclear RNA precursors to the mRNA for rat cytosolic phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32 ) and nuclear RNA species smaller in size than the mature enzyme mRNA have been identified by using hybridization probes specific for introns and exons from the gene. A number of processing intermediates, including an intact intron, were noted. Based on the size and distribution of the precursors observed, we propose a pathway for phosphoenolpyruvate carboxykinase RNA processing that involves splicing of introns starting at the 5' end of the RNA transcript and proceeding toward the 3' end.
