期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1988
卷号：85
期号：8
页码：2633-2637
DOI：10.1073/pnas.85.8.2633
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A mutant of Drosophila melanogaster carrying the lethal(1) myospheroid mutation [l(1)mys] has a defective musculature and a phenotype that suggests a defect of basement membranes. The genomic region that is interrupted by an insertion in a mutant carrying l(1)mys was used to isolate cDNA clones, and their sequences are presented here. The cDNA sequence predicts a cysteine-rich integral membrane protein that displays 45% sequence identity to chicken integrin and the human fibronectin receptor beta subunit and much greater similarity over localized segments. These similarities extend to other vertebrate integrin beta subunits, and we conclude that the myospheroid protein is an integrin beta subunit of Drosophila. This implies evolutionary conservation of a group of transmembrane proteins that are receptors for extracellular matrix and, coupled with the myospheroid phenotype, indicates an important role for the interaction of cells with extracellular matrix during development.
