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  • 标题:Photoaffinity labeling of indole-3-acetic acid-binding proteins in maize
  • 本地全文:下载
  • 作者:Alan M. Jones ; Michael A. Venis
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1989
  • 卷号:86
  • 期号:16
  • 页码:6153-6156
  • DOI:10.1073/pnas.86.16.6153
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The photoaffinity labeling agent 5-azidoindole-3-acetic acid, an analog of the endogenous plant hormone indole-3-acetic acid (an auxin), was used to identify indole-3-acetic acid-binding proteins in maize. Two peptides with subunit molecular masses of 24 and 22 kilodaltons are specifically labeled in a saturable manner. Both peptides are slightly acidic and behave as dimers under nondenaturing conditions. The possibility that one of these peptides is the auxin receptor that mediates cell elongation in maize is discussed.
  • 关键词:auxin receptor ; 5-azidoindole-3-acetic acid
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