首页    期刊浏览 2024年11月29日 星期五
登录注册

文章基本信息

  • 标题:Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro
  • 本地全文:下载
  • 作者:C Dingwall ; I Ernberg ; M J Gait
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1989
  • 卷号:86
  • 期号:18
  • 页码:6925-6929
  • DOI:10.1073/pnas.86.18.6925
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.
国家哲学社会科学文献中心版权所有