期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：65
期号：3
页码：683-690
DOI：10.1073/pnas.65.3.683
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Phospholipase D (cabbage) inhibits the vectorial phosphorylation of [α]-methylglucoside by isolated membrane preparations from Escherichia coli ML 308-225 without increasing the efflux of intramembranal [α]-methylglucoside-P. This effect is shown to be related to the ability of phospholipase D to hydrolyze membrane phosphatidylglycerol specifically. After treatment with phospholipase D, the membranes resynthesize phosphatidylglycerol with a return in their ability to take up [α]-methylglucoside. Since proline uptake by the same preparations is only slightly inhibited by phospholipase D, the data indicate that phosphatidylglycerol is required specifically for transport processes which are mediated by the P-enolpyruvate-P-transferase system.
