期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：65
期号：3
页码：737-744
DOI：10.1073/pnas.65.3.737
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A procedure is described for the selection of temperature-sensitive phospholipid mutants based upon radiation suicide of the wild-type organisms by tritiated L-glycerol-3-phosphate incorporated in the phospholipids. One of these mutants possesses an acyltransferase activity much more thermolabile than that of its parent. This mutant ceases growth and phospholipid biosynthesis immediately upon shift to a nonpermissive temperature although DNA, RNA, and protein continue to be synthesized. The phenotype of this mutant appears due to a single mutation by reversion analysis and by enzymatic analysis of temperature-resistant revertants.
