期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：66
期号：4
页码：1183-1189
DOI：10.1073/pnas.66.4.1183
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mouse myeloma cells pulse-labeled in vitro with 3H-leucine or 3H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.
