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  • 标题:β-GALACTOSIDE TRANSPORT IN BACTERIAL MEMBRANE PREPARATIONS: ENERGY COUPLING VIA MEMBRANE-BOUND D-LACTIC DEHYDROGENASE
  • 本地全文:下载
  • 作者:Eugene M. Barnes ; H. R. Kaback
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1970
  • 卷号:66
  • 期号:4
  • 页码:1190-1198
  • DOI:10.1073/pnas.66.4.1190
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:The transport of {beta}-galactosides by isolated membrane preparations from Escherichia coli strains containing a functional y gene is markedly stimulated by the conversion of D-lactate to pyruvate. The addition of D-lactate to these membrane preparations produces a 19-fold increase in the initial rate of uptake and a 10-fold stimulation of the steady-state level of intramembranal lactose or thiomethylgalactoside. Succinate, DL-[α]-hydroxybutyrate, and L-lactate partially replace D-lactate, but are much less effective; ATP and P-enolpyruvate, in addition to a number of other metabolites and cofactors, do not stimulate lactose transport by the vesicles. Lactose uptake by the membrane preparations in the presence of D-lactate requires oxygen, and is blocked by electron transport inhibitors and proton conductors; however, uptake is not significantly inhibited by high concentrations of arsenate or oligomycin. Furthermore, the P-enolpyruvate-P-transferase system is not involved in {beta}-galactoside transport by the E. coli membrane vesicles. The findings indicate that the {beta}-galactoside uptake system is coupled to the membrane-bound D-lactic dehydrogenase via an electron transport chain but does not involve oxidative phosphorylation.
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