期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1970
卷号:66
期号:4
页码:1190-1198
DOI:10.1073/pnas.66.4.1190
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:The transport of {beta}-galactosides by isolated membrane preparations from Escherichia coli strains containing a functional y gene is markedly stimulated by the conversion of D-lactate to pyruvate. The addition of D-lactate to these membrane preparations produces a 19-fold increase in the initial rate of uptake and a 10-fold stimulation of the steady-state level of intramembranal lactose or thiomethylgalactoside. Succinate, DL-[α]-hydroxybutyrate, and L-lactate partially replace D-lactate, but are much less effective; ATP and P-enolpyruvate, in addition to a number of other metabolites and cofactors, do not stimulate lactose transport by the vesicles. Lactose uptake by the membrane preparations in the presence of D-lactate requires oxygen, and is blocked by electron transport inhibitors and proton conductors; however, uptake is not significantly inhibited by high concentrations of arsenate or oligomycin. Furthermore, the P-enolpyruvate-P-transferase system is not involved in {beta}-galactoside transport by the E. coli membrane vesicles. The findings indicate that the {beta}-galactoside uptake system is coupled to the membrane-bound D-lactic dehydrogenase via an electron transport chain but does not involve oxidative phosphorylation.