期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：66
期号：4
页码：1252-1259
DOI：10.1073/pnas.66.4.1252
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Promitochondria of anaerobically grown Saccharomyces cerevisiae were selectively labeled in vivo by incubating the cells with [3H]leucine and cycloheximide. When the labeled cells were washed free of cycloheximide and adapted to oxygen in the presence of unlabeled leucine, the respiring mitochondria formed during adaptation proved to be radioactive. In contrast, only insignificant label was found in iso-1-cytochrome c which is synthesized de novo during adaptation. Respiratory adaptation of anaerobically grown yeast thus involves differentiation of promitochondrial organelles.
