期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：67
期号：2
页码：537-543
DOI：10.1073/pnas.67.2.537
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The protein factor S, previously shown to stimulate polypeptide chain termination in bacterial extracts, has two effects upon the complex formed between ribosomes, release factor, and terminator (trinucleotide) codon: (1) in the absence of GTP or GDP, S stimulates formation of an [R{middle dot}UAA{middle dot}ribosome] intermediate, and (2) in the presence of GTP or GDP, S participates in dissociation of this intermediate. Factor S can stimulate fMet release from [fMet-tRNAf{middle dot}AUG{middle dot}ribosome] intermediates in either the presence or absence of GTP or GDP. A model is proposed which relates the in vitro effects of S {+/-} GTP (or GDP) on fMet release to the effects of S {+/-} GTP (or GDP) on the binding and dissociation of R factor from ribosomes.
