期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1970
卷号：67
期号：2
页码：943-950
DOI：10.1073/pnas.67.2.943
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA from Mycoplasma sp. Kid which was enriched for tRNA genes (containing about 10% tDNA) was transcribed by E. coli RNA polymerase. The RNA transcription product labeled with [14C]uridine was formed in good yield (70-fold net synthesis). After incubation of this [14C]uridine-labeled RNA with E. coli extracts, nucleotide analyses revealed that [14C]pseudouridine was formed. The experiments support the idea that the conversion of uridine to pseudouridine takes place at the macromolecular level. Furthermore, the conversion was shown to be specific for a uridine residue in tRNA-like material since neither [14C]polyuridylic acid nor the [14C]uridine-labeled RNA transcribed from {lambda} DNA served as substrate for the pseudouridine-forming enzyme(s).
