期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：5
页码：938-941
DOI：10.1073/pnas.68.5.938
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A hydrophobic probe, 2-p-toluidinylnaphthalene-6-sulfonate (TNS), was introduced into the interior of a squid giant axon and fluorescene properties of the TNS-stained axon were examined under illumination with polarized ultraviolet light. A transient reduction in fluorescence was observed when the axon was stimulated electrically. The light waves contributing to this fluorescence change were found to be nearly completely polarized, indicating that the probe molecules in the membrane are highly oriented. The emission spectra of these TNS-stained axons were examined at rest and during nerve excitation. The results obtained are considered to support the view that the process of nerve excitation is accompanied by a transition of the axon membrane from a hydrophobic to a hydrophilic state.
