期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：6
页码：1174-1177
DOI：10.1073/pnas.68.6.1174
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The bacteriophage P1 infects and functions as a generalized transducing phage for nitrogen-fixing strains of the coliform bacterium Klebsiella pneumoniae. Bacterial mutants (nif-) unable to grow on molecular nitrogen as a nitrogen source were found to be deficient in nitrogenase activity as assayed by the conversion of acetylene to ethylene. These mutants regained normal nitrogenase activity and the ability to grow on N2 after transduction with lysates of P1 phage prepared from wild-type bacteria. Transductional analysis with P1 revealed that several nif genes are located on the genetic linkage map of Klebsiella near the histidine operon.
