期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：6
页码：1376-1380
DOI：10.1073/pnas.68.6.1376
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：RNA extracted from T4 phage-infected Escherichia coli cells can direct the synthesis of phage-specific deoxynucleotide kinase in a cell-free system from uninfected E. coli. The maximum messenger activity is obtained 8 min after infection at 37{degrees}C and decreases there-after. The main activity of this messenger RNA has a sedimentation coefficient of about 15 S on a sucrose density gradient. The conditions necessary for the appearance of enzyme activity in vitro are the same as those required for de novo synthesis of protein. RNA from cells infected with a T4 amber mutant incapable of inducing the kinase in vivo lacks the ability to direct the synthesis of enzyme in vitro.
