期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：7
页码：1416-1420
DOI：10.1073/pnas.68.7.1416
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A method is described for determining the two-dimensional distribution of specific antigens on cell surfaces, and is applied to the D antigen of the Rh antigenic system. Rh-positive human erythrocytes are allowed to react with purified 125I-labeled human anti-Rho(D) {gamma}-globulin antibodies, and the sensitized cells are then lysed at an air-water interface. The residual cell membranes are spread flat by surface forces, and are picked up on a carbon-strengthened collodion-coated electron microscope grid. The membranes are then stained with ferritin-conjugated goat antibodies directed against human {gamma}-globulins. Only Rh-positive cells sensitized with anti-Rho(D) antibodies bind the ferritin-conjugated antihuman {gamma}-globulins. The ferritin particles are found in small clusters on the membrane surface, and the number of such clusters per unit area agrees with the number of 125I-labeled anti-Rho(D) antibodies bound per unit area. The Rho(D) antigenic sites appear to be molecularly dispersed on the membrane surface, but in a random two dimensional array.
