期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：8
页码：1805-1809
DOI：10.1073/pnas.68.8.1805
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The initiator tRNA, methionyl-tRNAiMet, of yeast and wheat germ forms relatively unstable ternary complexes with their corresponding elongation factors T and GTP. Such complexes can be demonstrated only with fast separation techniques such as Sephadex G-50 and Millipore filtration, but not with the slow Sephadex G-100 method, although both techniques yield stable ternary complexes with all other aminoacyl-tRNAs, including the internal Met-tRNAmMet. To bind yeast-initiating Met-tRNAiMet to ribosomes, initiation factors present in a ribosomal wash fraction from yeast are needed.
