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  • 标题:Stimulation of RNA Transcription from Pea and Corn DNA by Protein Retained on Sepharose Coupled to 2,4-Dichlorophenoxyacetic Acid
  • 本地全文:下载
  • 作者:Michael A. Venis
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1971
  • 卷号:68
  • 期号:8
  • 页码:1824-1827
  • DOI:10.1073/pnas.68.8.1824
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.
  • 关键词:E. coli polymerase
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