期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：9
页码：2037-2041
DOI：10.1073/pnas.68.9.2037
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The binding of alkali cations by the ionophorous antibiotics valinomycin, nigericin, alamethicin, and the macrotetralide actins has been shown to occur, in aqueous media, by the use of the fluorescent probes 1-anilino-8-naphthalene sulfonate and 2-p-toluidinyl-6-naphthalene sulfonate. The interaction of the ionophore-cation complexes with the fluorescent dyes produced enhanced fluorescence emission, increased lifetime and polarization, and a significant blue-shift of the emission maxima of the fluorescence spectrum. At constant antibiotic and fluorophore concentrations in water, the intensity of the fluorescence emission was found to be a function of the cation concentration. This permitted relative cation affinities to be determined for alamethicin (Na+ {cong} K+), valinomycin (Rb+ > K+ > Cs+), nigericin (K+ > Rb+ > Na+ > Cs+) and trinactin (NH4+ > K+ > Rb+ > Cs+).
