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  • 标题:Determination of the Gene Sequence of Poliovirus with Pactamycin
  • 本地全文:下载
  • 作者:D. F. Summers ; J. V. Maizel
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1971
  • 卷号:68
  • 期号:11
  • 页码:2852-2856
  • DOI:10.1073/pnas.68.11.2852
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:By examination of the virus-specific polypeptides formed after the addition of pactamycin, an inhibitor of protein chain initiation, to infected cells, it has been possible to tentatively locate the virus coat proteins at the amino terminus of the large, virus-specific protein precursor, and, therefore, to assign the coat protein cistron to the 5' end of the RNA.
  • 关键词:translation ; HeLa cells ; protein precursor molecules ; gel electrophoresis ; initiation
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