期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：11
页码：2830-2834
DOI：10.1073/pnas.68.11.2830
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mitochondrial RNA polymerase activity from rat liver has previously been demonstrated in intact organelles. This activity has now been solubilized, partially purified, and shown to be a true polymerase, free of nuclease. The enzyme is derived from mitochondria and is not from contaminating bacteria or nuclear components. The enzyme is distinguished from its nuclear counterparts by its behavior on ammonium sulfate fractionation and lack of inhibition by [α]-amanitin. Rifamycin inhibits the crude enzyme, but only inconsistently inhibits the more purified preparation.
